| |
|
|
| Registros recuperados : 28 | |
| 4. |  | SILVEIRA, E. de A.; TARDIOLI, P. W.; FARINAS, C. S. Imobilização de lipase de Aspergillus niger em suportes hidrofóbicos. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2018. p. 85. Editores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva. (Embrapa Instrumentação. Documentos, 68). (Embrapa Instrumentação. Documentos, 68).| Biblioteca(s): Embrapa Instrumentação. |
|    |
| 5. | | COUTINHO, T. C.; TARDIOLI, P. W.; FARINAS, C. S. Melhoria nas propriedades da fitase após sua imobilização em hidroxiapatita para aplicação em ração animal. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 570-574| Biblioteca(s): Embrapa Instrumentação. |
| |
| 8. | | SILVEIRA, E. A.; TARDIOLI, P. W.; FARINAS, C. S. Screening de fungos lipolíticos e produção de lipases por fermentação em estado sólido utilizando resíduos agroindustriais do processamento do dendê. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 501-504. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.| Biblioteca(s): Embrapa Instrumentação. |
| |
| 10. | | SILVEIRA, E. A.; TARDIOLI, P. W.; FARINAS, C. S. Triagem de fungos lipolíticos para produção enzimática utilizando o processo de fermentação combinada. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 6., 2014, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2014. (Embrapa Instrumentação. Documentos, 57). Editores técnicos: João de Mendonça Naime, Caue Ribeiro, Maria Alice Martins, Elaine Cristina Paris, Paulino Ribeiro Villas Boas, Ladislau Marcelino Rabello. p. 22.| Biblioteca(s): Embrapa Instrumentação. |
| |
| 11. | | VASCONCELLOS, V. M.; TARDIOLI, P. W.; GIORDANO, R. L. C.; FARINAS, C. S. Addition of metal ions to a (hemi)cellulolytic enzymatic cocktail produced in-house improves its activity, thermostability, and efficiency in the saccharification of pretreated sugarcane bagasse. New biotechnology, [S. l.], v. 33, n. 3, p. 331-337, 2016.| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 14. | | BARALDO, A.; BORGES, D. G.; FARINAS, C. S.; TARDIOLI, P. W. Caracterização de B-glicosidade produzida por Aspergillus niger quanto ao pH e temperatura. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 51. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Biblioteca(s): Embrapa Instrumentação. |
| |
| Registros recuperados : 28 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
23/03/2022 |
|
Data da última atualização: |
24/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
COUTINHO, T. C.; MALAFATTI, J. O. D.; PARIS, E. C.; TARDIOLI, P. W.; FARINAS, C. S. |
|
Afiliação: |
ELAINE CRISTINA PARIS, CNPDIA; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
|
Título: |
Hydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
ACS Applied Nano Materials, v. 3, 2020. |
|
Páginas: |
12334–12345 |
|
DOI: |
https://dx.doi.org/10.1021/acsanm.0c02811 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Hydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining MenosHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes... Mostrar Tudo |
|
Palavras-Chave: |
Cobalt ferrite; Immobilization; Magnetic nanoparticles. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02369naa a2200229 a 4500
001 2141192
005 2024-01-24
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://dx.doi.org/10.1021/acsanm.0c02811$2DOI
100 1 $aCOUTINHO, T. C.
245 $aHydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery.$h[electronic resource]
260 $c2022
300 $a12334–12345
520 $aHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining
653 $aCobalt ferrite
653 $aImmobilization
653 $aMagnetic nanoparticles
700 1 $aMALAFATTI, J. O. D.
700 1 $aPARIS, E. C.
700 1 $aTARDIOLI, P. W.
700 1 $aFARINAS, C. S.
773 $tACS Applied Nano Materials$gv. 3, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
